Fibrillarin antibody
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- Target See all Fibrillarin (FBL) Antibodies
- Fibrillarin (FBL)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Fibrillarin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- IP/MS validation was supported by references (PMID:30377378)
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to Fibrillarin (fibrillarin)
Fibrillarin antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
- Isotype
- IgG
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: Rat2 , 293T , H1299 , NIH-3T3
Validation: IP/MS
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- Fibrillarin (FBL)
- Alternative Name
- fibrillarin (FBL Products)
- Synonyms
- FIB antibody, FLRN antibody, RNU3IP1 antibody, wu:fb37g12 antibody, zgc:56145 antibody, zgc:77130 antibody, FBL antibody, AL022665 antibody, CG9888 antibody, Dmel\\CG9888 antibody, Fibri antibody, GCR-6 antibody, GCR6 antibody, Pen59C5 antibody, fib antibody, pen59C5 antibody, MGC76139 antibody, fbl antibody, fibrillarin antibody, Fibrillarin antibody, putative fibrillarin antibody, fibrillarin S homeolog antibody, FBL antibody, fbl antibody, Fbl antibody, Fib antibody, fib1 antibody, LMJF_19_0100 antibody, fbl.S antibody
- Background
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This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.
Cellular Localization: Nucleus , nucleolus - Molecular Weight
- 34 kDa
- Gene ID
- 2091
- UniProt
- P22087
- Pathways
- Ribonucleoside Biosynthetic Process
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