FEN1 antibody

Catalog No. ABIN2855166

Product Details anti-FEN1 Antibody

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to FEN1 (flap structure-specific endonuclease 1)
FEN1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: flap structure-specific endonuclease 1 (FEN1 Products)

Synonyms: FEN-1 antibody, FEN1 antibody, AW538437 antibody, MF1 antibody, RAD2 antibody, cb879 antibody, fen-1 antibody, fen1 antibody, mf1 antibody, rad2 antibody, xFEN1a antibody, flap structure-specific endonuclease 1 antibody, flap endonuclease 1-like antibody, Flap endonuclease-1 antibody, 5'-3' exonuclease family protein antibody, flap structure specific endonuclease 1 antibody, flap structure-specific endonuclease 1 L homeolog antibody, nuclease, Rad2 family antibody, FEN1 antibody, LOC100808044 antibody, Bm1_49605 antibody, AT5G26680 antibody, Fen1 antibody, fen1 antibody, fen1.L antibody

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Cellular Localization: Nucleus

Molecular Weight: 43 kDa

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA