PAX8 antibody

Catalog No. ABIN2855097

Product Details anti-PAX8 Antibody

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity: Dog, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PAX8 (paired box 8)
PAX8 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T nuclear extract , DDDDK-tagged PAX8-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PAX8 antibody (ABIN2855097)

Shi, Yin, Cai, Yan, Jia, Ma, Zhang, Zhang, Gu, Zhang, Di, Zhuang: "PAX8 regulon in human ovarian cancer links lineage dependency with epigenetic vulnerability to HDAC inhibitors." in: eLife, Vol. 8, (2020) (PubMed).

Sun, Lu, Wu, Ouyang, Xing, Hou, Shi, Wu: "PTEN-knockdown disrupts the morphology, growth pattern and function of Nthy-Ori 3-1 cells by downregulating PAX8 expression." in: Oncology letters, Vol. 18, Issue 6, pp. 6732-6740, (2019) (PubMed).

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: paired box 8 (PAX8 Products)

Synonyms: PAX8 antibody, XPax-8 antibody, XPax8 antibody, pax-8 antibody, PAX-8 antibody, Pax-8 antibody, paired box 8 antibody, paired box 8 L homeolog antibody, PAX8 antibody, pax8 antibody, Pax8 antibody, pax8.L antibody

Background: This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Nucleus

Molecular Weight: 48 kDa

Gene ID: 7849

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation