CD19 antibody

Catalog No. ABIN2855077

Product Details anti-CD19 Antibody

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CD19 (CD19 Molecule)
CD19 antibody [C1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD19. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse leukocyte

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.21 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CD19 antibody (ABIN2855077)

Kurimoto, Inaba, Ariyasu, Iwakura, Ueda, Uraki, Takeshima, Furukawa, Morita, Yamamoto, Yamashita, Katsuda, Hayata, Akamatsu, Jinnin, Hara, Yamaue, Akamizu: "Predictive and sensitive biomarkers for thyroid dysfunctions during treatment with immune-checkpoint inhibitors." in: Cancer science, Vol. 111, Issue 5, pp. 1468-1477, (2020) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 molecule (CD19 Products)

Background: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Molecular Weight: 61 kDa

Gene ID: 930

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway