BCL6 antibody (Internal Region)

Catalog No. ABIN2855012

Product Details anti-BCL6 Antibody

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Characteristics: Rabbit Polyclonal antibody to BCL6 (B-cell CLL/lymphoma 6)
BCL6 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ChIP assay Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: Jurkat , Raji , NCI-H929 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: BCL6 (BCL6 Products)

Synonyms: bcl5 antibody, laz3 antibody, bcl6a antibody, znf51 antibody, zbtb27 antibody, MGC132260 antibody, Bcl5 antibody, BCL5 antibody, BCL6A antibody, LAZ3 antibody, ZBTB27 antibody, ZNF51 antibody, B-cell CLL/lymphoma 6 L homeolog antibody, B-cell CLL/lymphoma 6 antibody, B cell leukemia/lymphoma 6 antibody, bcl6.L antibody, bcl6 antibody, BCL6 antibody, Bcl6 antibody

Background: The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 79 kDa

Gene ID: 604

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus