TUBB antibody

Catalog No. ABIN2854998

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Hamster, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta)
beta Tubulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.44 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TUBB antibody (ABIN2854998)

Wakae, Nishiyama, Kondo, Izuka, Que, Chen, Kase, Kitamura, Mohiuddin, Wang, Ahasan, Nakamura, Fujiwara, Yoshizaki, Hosomochi, Tajima, Nakahara, Kiyono, Muramatsu: "Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation." in: Scientific reports, Vol. 8, Issue 1, pp. 9745, (2019) (PubMed).

Lin, Ho, Lin, Hsu, Huang, Liao, Lai, Liu, Tsang, Lai, Tsai: "P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions." in: BioMedicine, Vol. 5, Issue 2, pp. 10, (2015) (PubMed).

Kohl, Zobor, Chiang, Weisschuh, Staller, Gonzalez Menendez, Chang, Beck, Garcia Garrido, Sothilingam, Seeliger, Stanzial, Benedicenti, Inzana, Héon, Vincent, Beis, Strom, Rudolph, Roosing, Hollander et al.: "Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia. ..." in: Nature genetics, Vol. 47, Issue 7, pp. 757-65, (2015) (PubMed).

Lin, Chen, Jeang, Liu, Wang, Hung, Tsang, Lin, Liao, Huang, Lin, Ho, Chien, Chen, Ho, Tsai: "Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture." in: Cell & bioscience, Vol. 4, pp. 40, (2014) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta Tubulin (TUBB Products)

Gene ID: 203068

Pathways: Microtubule Dynamics, M Phase