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Hsc70 antibody (C-Term)

Name: Hsc70 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2854950
Price: 637.78 USD
Availability: InStock

HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (Hsc70 antibody (C-Term))

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using HSC70, antibody at 1:200 dilution. (Hsc70 antibody (C-Term))

IHC-P Image HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis. (Hsc70 antibody (C-Term))

IHC

IHC-P Image HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis. (Hsc70 antibody (C-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70, antibody at 1:100 dilution. (Hsc70 antibody (C-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: H1299 7. (Hsc70 antibody (C-Term))

6 images

Catalog No. ABIN2854950

Datasheet as PDF

Target See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Binding Specificity
14

7

6

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
77

41

37

16

12

9

8

6

6

6

5

5

5

4

4

3

3

2

1

1

1

1

1

1

1
Human, Rat, Mouse
Host
68

27

5

2
Rabbit
Compatible Secondaries
Clonality
66

35
Polyclonal
Conjugate
56

18

11

3

2

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
Application
89

51

38

28

27

27

12

11

11

11

11

5

4

2

2

2
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Pig (Porcine), Rhesus Monkey

Cross-Reactivity (Details)

Pig (100 %), Rhesus Monkey (100 %)

Characteristics

Rabbit Polyclonal antibody to Hsc70 (heat shock 70 kDa protein 8)
HSC70 antibody

Purification

Affinity purified by Protein A.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HSC70. The exact sequence is proprietary.

Isotype

IgG

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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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HSPA8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Restrictions

For Research Use only
Format

Liquid

Concentration

10 mg/mL

Buffer

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSC70 (HSPA8 Products)

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.

Cellular Localization: Cytoplasm , Melanosome

Molecular Weight

71 kDa

Gene ID

3312