PPARA antibody (N-Term)

Catalog No. ABIN2854934

Product Details anti-PPARA Antibody

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity: Bee, Human, Mouse, Plant, Rat

Characteristics: Rabbit Polyclonal antibody to PPAR alpha (peroxisome proliferator-activated receptor alpha)
PPAR alpha antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.39 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PPARA antibody (ABIN2854934)

Kubo, Hoshi, Matsumoto, Irie, Oura, Tsutsumi, Hata, Yamamoto, Saito: "Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice." in: Lipids in health and disease, Vol. 16, Issue 1, pp. 106, (2018) (PubMed).

Song, Qu, Zhang, Jiang, Zhou, Jiang, Li, Zhang, Yan: "Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet." in: Scientific reports, Vol. 7, pp. 41604, (2018) (PubMed).

Chung, Liu, Lin, Chi, Huang, Yang, Yeh, Tan, Lin: "ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance." in: Oncotarget, Vol. 7, Issue 16, pp. 22448-59, (2017) (PubMed).

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: peroxisome proliferator activated receptor alpha (PPARA Products)

Synonyms: NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, LOC100136415 antibody, PPARALPHA antibody, nr1c1 antibody, xPPARalpha antibody, xppara antibody, 4933429D07Rik antibody, AW742785 antibody, Nr1c1 antibody, PPAR-alpha antibody, Ppar antibody, peroxisome proliferator activated receptor alpha antibody, peroxisome proliferator-activated receptor alpha antibody, peroxisome proliferator activated receptor alpha L homeolog antibody, PPARA antibody, LOC100136415 antibody, ppara.L antibody, Ppara antibody, ppara antibody

Background: Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers, this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.

Molecular Weight: 52 kDa

Gene ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C