ALPL antibody (Center)

Catalog No. ABIN2854870

Product Details anti-ALPL Antibody

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Binding Specificity: Center

Reactivity: Human, Mouse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Cat (Feline), Dog (Canine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (91 %), Cat (90 %), Dog (89 %), Rat (91 %), Bovine (89 %)

Characteristics: Rabbit polyclonal antibody to alkaline phosphatase (liver/bone/kidney) (alkaline phosphatase, liver/bone/kidney)
Alkaline Phosphatase (Tissue Non-Specific) antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ALPL antibody (ABIN2854870)

Agustina, Asyifa, Aziz, Hernowo: "The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis." in: Pathology research international, Vol. 2018, pp. 6346409, (2018) (PubMed).

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline Phosphatase (Liver/bone/kidney) (ALPL Products)

Synonyms: ALP antibody, iap antibody, alpl antibody, AP-TNAP antibody, APTNAP antibody, HOPS antibody, TNAP antibody, TNSALP antibody, Akp-2 antibody, Akp2 antibody, PHOA antibody, TNS-AP antibody, alkaline phosphatase, liver/bone/kidney L homeolog antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase, intestinal, gene 2 antibody, alkaline phosphatase, liver/bone/kidney pseudogene antibody, alpl.L antibody, ALPL antibody, alpi.2 antibody, LOC100031702 antibody, Alpl antibody

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Cellular Localization: Cell membrane, Lipid-anchor , GPI-anchor

Molecular Weight: 57 kDa

Gene ID: 249