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c-MET antibody (C-Term)

Name: c-MET antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2854819
Price: 637.78 USD
Availability: InStock

MET Reactivity: Human WB, IF, ICC, IP Host: Rabbit Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:HeLa S3, 5% SDS PAGE antibody diluted at 1:1000 (c-MET antibody (C-Term))

ICC/IF Image c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis. (c-MET antibody (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using c-Met, antibody at 1:100 dilution. (c-MET antibody (C-Term))

IHC

IP Image Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term, Western blot analysis was performed using c-Met antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (c-MET antibody (C-Term))

MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. (c-MET antibody (C-Term))

5 images

Catalog No. ABIN2854819

Datasheet as PDF

Target See all c-MET (MET) Antibodies

c-MET (MET) (Met Proto-Oncogene (MET))
Binding Specificity
42

28

28

28

11

11

9

7

7

7

6

5

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
229

134

102

6

6

5

4

4

2

2

2

1

1

1

1
Human
Host
200

39

3

2
Rabbit
Compatible Secondaries
Clonality
196

48
Polyclonal
Conjugate
127

19

11

11

7

7

7

7

7

7

6

6

2

2

2

2

2

2

2

2

2

2

2
This c-MET antibody is un-conjugated
Application
161

103

43

28

26

19

15

14

11

8

8

5

4

4

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity

Chimpanzee

Cross-Reactivity (Details)

Chimpanzee (100 %)

Characteristics

Rabbit Polyclonal antibody to MET (met proto-oncogene (hepatocyte growth factor receptor))
c-Met antibody [C3], C-term

Purification

Purified by antigen-affinity chromatography.

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Isotype

IgG

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MET Reactivity: Human, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (AA 743-932) antibody
MET Reactivity: Human WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 5E9C4 unconjugated

anti-Met Proto-Oncogene (MET) (AA 1092-1379) antibody
MET Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) antibody
MET Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Monoclonal 2D12 unconjugated Recombinant Antibody

anti-Met Proto-Oncogene (MET) (AA 743-932) antibody
MET Reactivity: Human ELISA, FACS, ICC Host: Mouse Monoclonal 5E9B7 unconjugated

anti-Met Proto-Oncogene (MET) (Internal Region) antibody
MET Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (Tyr1003) antibody
MET Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (AA 83-209) antibody
MET Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (pTyr1234) antibody
MET Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (pTyr1003) antibody
MET Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment

Positive Control: HeLaS3

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name

C-Met (MET Products)

Synonyms

AUTS9 antibody, HGFR antibody, RCCP2 antibody, c-Met antibody, AI838057 antibody, HGF antibody, Par4 antibody, Hgfr antibody, c-met antibody, MET antibody, C-MET antibody, met antibody, met-A antibody, MET proto-oncogene, receptor tyrosine kinase antibody, met proto-oncogene antibody, MET proto-oncogene, receptor tyrosine kinase L homeolog antibody, MET antibody, Met antibody, met.L antibody

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Membrane, Single-pass type I membrane protein

Molecular Weight

156 kDa

Gene ID

4233

Pathways

RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor