POLG antibody (C-Term)
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- Target See all POLG Antibodies
- POLG (Polymerase (DNA Directed), gamma (POLG))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLG antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Cross-Reactivity
- Human, Rat
- Characteristics
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Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)
DNA polymerase gamma antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.
- Isotype
- IgG
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: Rat testis , MCF-7 , MDA-MB-231
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.67 mg/mL
- Buffer
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- POLG (Polymerase (DNA Directed), gamma (POLG))
- Alternative Name
- DNA polymerase gamma (POLG Products)
- Synonyms
- POLG antibody, MDP1 antibody, MIRAS antibody, MTDPS4A antibody, MTDPS4B antibody, PEO antibody, POLG1 antibody, POLGA antibody, SANDO antibody, SCAE antibody, AA409516 antibody, PolgA antibody, polymerase (DNA) gamma, catalytic subunit L homeolog antibody, DNA polymerase gamma, catalytic subunit antibody, polymerase (DNA directed), gamma antibody, polg.L antibody, Polg antibody, POLG antibody, polg antibody
- Target Type
- Viral Protein
- Background
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Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
Cellular Localization: Mitochondrion - Molecular Weight
- 140 kDa
- Gene ID
- 5428
- UniProt
- P54098
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