HRH4 antibody (N-Term)

Catalog No. ABIN2854739

Product Details anti-HRH4 Antibody

Target: HRH4 (Histamine Receptor H4 (HRH4))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH4 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to Histamine H4 Receptor (histamine receptor H4)
Histamine H4 Receptor antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histamine H4 Receptor. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , NCI-H929 , human shHRH4-transfected HeLa cells

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 50 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HRH4 antibody (ABIN2854739)

Adderley, Zhang, Breslin: "Involvement of the H1 Histamine Receptor, p38 MAP Kinase, Myosin Light Chains Kinase, and Rho/ROCK in Histamine-Induced Endothelial Barrier Dysfunction." in: Microcirculation (New York, N.Y. : 1994), Vol. 22, Issue 4, pp. 237-48, (2016) (PubMed).

Target Details for HRH4

Target: HRH4 (Histamine Receptor H4 (HRH4))

Alternative Name: histamine receptor H4 (HRH4 Products)

Background: Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors.

Cellular Localization: Cell membrane, Multi-pass membrane protein

Molecular Weight: 44 kDa

Gene ID: 59340

UniProt: Q9H3N8