CXCR5 antibody

Catalog No. ABIN2854723

Product Details anti-CXCR5 Antibody

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CXCR5 (chemokine (C-X-C motif) receptor 5)
CXCR5 antibody [C1C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CXCR5 antibody (ABIN2854723)

Lin, Izar, Wang, Yapp, Mei, Shah, Santagata, Sorger: "Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes." in: eLife, Vol. 7, (2019) (PubMed).

Yang, Wei, Liu, Shi, Li: "Frequency and distribution of CD4+CXCR5+ follicular B helper T cells within involved tissues in IgG4‑related ophthalmic disease." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 9512-9520, (2018) (PubMed).

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: C-X-C motif chemokine receptor 5 (CXCR5 Products)

Synonyms: BLR1 antibody, CRL1 antibody, CD185 antibody, MDR15 antibody, Blr1 antibody, CXC-R5 antibody, CXCR-5 antibody, Gpcr6 antibody, NLR antibody, C-X-C motif chemokine receptor 5 antibody, chemokine (C-X-C motif) receptor 5 antibody, CXCR5 antibody, Cxcr5 antibody

Background: This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist.

Cellular Localization: Cell membrane

Molecular Weight: 42 kDa

Gene ID: 643

UniProt: P32302