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beta Actin antibody (N-Term)

Name: beta Actin antibody (N-Term)
Brand: antibodies-online
SKU: ABIN2854709
Price: 637.78 USD
Availability: InStock

KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody antibody diluted at 1:1000 (beta Actin antibody (N-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin, antibody (Green) at 1:1000 dilution. (beta Actin antibody (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin, antibody at 1:500 dilution. (beta Actin antibody (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (beta Actin antibody (N-Term))

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody , diluted at 1:1000. (beta Actin antibody (N-Term))

IHC-P Image beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. (beta Actin antibody (N-Term))

IHC

6 images

(4 references)

Catalog No. ABIN2854709

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
40

16

15

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
181

131

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Human
Host
115

82

5

1

1
Rabbit
Compatible Secondaries
Clonality
112

90
Polyclonal
Conjugate
129

13

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
171

74

54

46

32

30

26

22

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Cat, Dog, Drosophila melanogaster, Human, Mouse

Characteristics

Rabbit Polyclonal antibody to beta Actin (actin, beta)
beta Actin antibody

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Isotype

IgG

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ACTB Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human WB, IF, IP Host: Mouse Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: NIH-3T3 , Raji , 293T
Validation: KO/KD

Restrictions

For Research Use only
Format

Liquid

Concentration

1.16 mg/mL

Buffer

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Yu, Luan, Liu, Su, Li: "MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal." in: Pharmacology, Vol. 105, Issue 3-4, pp. 190-201, (2020) (PubMed).

Tao, Cheng, Yang: "Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway." in: Clinical interventions in aging, Vol. 15, pp. 373-385, (2020) (PubMed).

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

actin beta (ACTB Products)

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight

42 kDa

Gene ID

60

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction