Cortactin antibody (N-Term)

Catalog No. ABIN2854674

Product Details anti-Cortactin Antibody

Target: Cortactin (CTTN)

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Cortactin (src substrate cortactin)
Cortactin antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104334 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104334

Date: 04/20/2021

Antigen: Cortactin

Lot Number: 39568

Method validated: Multiplex Immunohistochemistry

Positive Control:

Fresh frozen mouse olfactory bulb

Negative Control:

Unlabeled control (mouse fresh frozen)

Notes:

Passed. The anti-cortactin antibody ABIN2854674 produces high degrees of labeling in granular and external plexiform layers of the mouse olfactory bulb. Additional labeling can be observed in the neuropil of individual glomeruli which is consistent with staining of synapses and neuronal processes.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-cortactin antibody ABIN2854674 (green; conjugated to fluorophore ATTO 550). Labeling is present in high degrees of labeling in granular and external plexiform layers with additional staining observed in the glomerular neuropil regions. Grin2b and doublecortin were labeled with ABIN5611338 (red; bound to fluorophore ATTO 550) and a doublecortin antibody (blue; bound to fluorophore AF 488).

FFPE normal human cortex tissue section labeled with anti-cortactin antibody ABIN2854674 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and neurofilament were additionally labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-neurofilament antibody (blue; bound to fluorophore AF488).

Full Methods

Primary Antibody: ABIN2854674

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-cortactin ABIN2854674 antibody to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Experimental Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: cortactin (CTTN Products)

Synonyms: EMS1 antibody, 1110020L01Rik antibody, Ems1 antibody, Cttnb antibody, CG3637 antibody, Dmel\CG3637 antibody, cortactin antibody, cttn antibody, CTTN1 antibody, P85.25 antibody, Cttn antibody, ems1 antibody, CTTN antibody, cortactin antibody, CG3637 gene product from transcript CG3637-RD antibody, cortactin S homeolog antibody, cortactin L homeolog antibody, src substrate cortactin antibody, Src substrate cortactin antibody, CTTN antibody, Cttn antibody, Cortactin antibody, cttn.S antibody, cttn.L antibody, cttn antibody, CpipJ_CPIJ006351 antibody, Bm1_57220 antibody, LOC100533277 antibody

Background: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Cellular Localization: Cytoplasm , cytoskeleton (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity)

Molecular Weight: 62 kDa

Gene ID: 2017

UniProt: Q14247

Pathways: MAPK Signaling