LC3B antibody

Catalog No. ABIN2854667

Product Details anti-LC3B Antibody

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to LC3B (microtubule-associated protein 1 light chain 3 beta)
LC3B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length rat LC3B Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

virus infected Huh7 , PC-3 , mouse brain , rat brain extract , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LC3B antibody (ABIN2854667)

Qi, Ren, Ba, Song, Zhang, Cao, Shi, Fu, Liu, Sun: "MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPARγ/NF-κB." in: Molecular therapy. Nucleic acids, Vol. 19, pp. 507-522, (2020) (PubMed).

Wei, Chen, Chen, Lou, Lin, Hung, Hsiao, Yao, Shieh: "Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells." in: Autophagy, Vol. 10, Issue 7, pp. 1179-92, (2015) (PubMed).

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: microtubule-associated protein 1 light chain 3 beta (MAP1LC3B Products)

Background: May mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD]

Cellular Localization: Cytoplasm , Endomembrane system, Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane, Lipid-anchor

Molecular Weight: 16 kDa

Gene ID: 64862

UniProt: Q62625

Pathways: Autophagy