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CENPF antibody (C-Term)

CENPF Reactivity: Human WB, IHC (p), IF, ICC, IHC (wm) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2854654
  • Target See all CENPF Antibodies
    CENPF (Centromere Protein F (CENPF))
    Binding Specificity
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 22
    • 2
    • 1
    • 1
    Human
    Host
    • 20
    • 1
    • 1
    Rabbit
    Clonality
    • 21
    • 1
    Polyclonal
    Conjugate
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CENPF antibody is un-conjugated
    Application
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))
    Cross-Reactivity
    Human, Zebrafish (Danio rerio)
    Characteristics
    Rabbit Polyclonal antibody to CENPF (centromere protein F, 350/400 kDa (mitosin))
    CENPF antibody [C3], C-term
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: Raji

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chen, Lin, Cheng, Lin, Lo, Yen, Lee, Hsiao: "Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition EMT in zebrafish." in: Oncotarget, Vol. 5, Issue 15, pp. 6300-11, (2015) (PubMed).

  • Target
    CENPF (Centromere Protein F (CENPF))
    Alternative Name
    centromere protein F (CENPF Products)
    Synonyms
    LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody
    Background
    This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

    Cellular Localization: Cytoplasm , perinuclear region , Nucleus matrix , Kinetochore , Spindle
    Molecular Weight
    368 kDa
    Gene ID
    1063
    UniProt
    P49454
    Pathways
    Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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