PPOX antibody

Catalog No. ABIN2854549

Product Details anti-PPOX Antibody

Target: PPOX (Protoporphyrinogen Oxidase (PPOX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPOX antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to PPOX (protoporphyrinogen oxidase)
PPOX antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PPOX. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.82 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPOX

Target: PPOX (Protoporphyrinogen Oxidase (PPOX))

Alternative Name: protoporphyrinogen oxidase (PPOX Products)

Background: This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

Cellular Localization: Mitochondrion inner membrane, Peripheral membrane protein, Intermembrane side

Molecular Weight: 51 kDa

Gene ID: 5498

UniProt: P50336

Pathways: Synaptic Membrane, Feeding Behaviour