ALAS2 antibody (Center)

Catalog No. ABIN2854459

Product Details anti-ALAS2 Antibody

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Rat (95 %), Xenopus laevis (81 %), Zebrafish (80 %), Bovine (95 %), Xenopus tropicalis (81 %)

Characteristics: Rabbit Polyclonal antibody to ALAS-E (aminolevulinate, delta-, synthase 2)
ALAS-E antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ALAS-E. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: Raji , K562 , HL60 , mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ALAS2 antibody (ABIN2854459)

Vercellotti, Khan, Nguyen, Chen, Bruzzone, Bechtel, Brown, Nath, Steer, Hebbel, Belcher: "H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice." in: Frontiers in pharmacology, Vol. 5, pp. 79, (2014) (PubMed).

Target Details for ALAS2

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Alternative Name: ALAS-E (ALAS2 Products)

Synonyms: anh1 antibody, asb antibody, xlsa antibody, ALAS-E antibody, ALASE antibody, ANH1 antibody, ASB antibody, XLDPP antibody, XLEPP antibody, XLSA antibody, alas-e antibody, cb1063 antibody, sau antibody, sauternes antibody, ALAS antibody, Alas-2 antibody, 5'-aminolevulinate synthase 2 antibody, aminolevulinate, delta-, synthase 2 antibody, aminolevulinic acid synthase 2, erythroid antibody, alas2 antibody, ALAS2 antibody, Alas2 antibody, alas2.L antibody

Background: The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 65 kDa

Gene ID: 212

Pathways: Transition Metal Ion Homeostasis