ZAK antibody

Catalog No. ABIN2854433

Product Details anti-ZAK Antibody

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAK antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to ZAK
ZAK antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human ZAK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ZAK

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Alternative Name: mitogen-activated protein kinase kinase kinase 20 (ZAK Products)

Background: This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 91 kDa

Gene ID: 51776

UniProt: Q9NYL2