LIG1 antibody (C-Term)

Catalog No. ABIN2854412

Product Details anti-LIG1 Antibody

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to DNA ligase I (ligase I, DNA, ATP-dependent)
DNA ligase I antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase I. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LIG1 antibody (ABIN2854412)

Liddiard, Ruis, Kan, Cleal, Ashelford, Hendrickson, Baird: "DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase." in: Nucleic acids research, Vol. 47, Issue 5, pp. 2402-2424, (2019) (PubMed).

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA ligase 1 (LIG1 Products)

Synonyms: ATLIG1 antibody, DNA ligase 1 antibody, T6D22.23 antibody, ligI antibody, AL033288 antibody, LigI antibody, lig1 antibody, wu:fc54a11 antibody, DNA ligase 1 antibody, DNA ligase 1, putative antibody, ligase I, DNA, ATP-dependent L homeolog antibody, ligase I, DNA, ATP-dependent antibody, LIG1 antibody, PB000674.02.0 antibody, PTRG_06243 antibody, PKH_140260 antibody, lig1.L antibody, Lig1 antibody, lig-1 antibody, lig1 antibody

Background: LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Cellular Localization: Nucleus

Molecular Weight: 102 kDa

Gene ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA