ATP6V1F antibody
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- Target See all ATP6V1F Antibodies
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATP6V1F antibody is un-conjugated
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Application
- Western Blotting (WB)
- Cross-Reactivity
- Human, Mouse
- Characteristics
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Rabbit Polyclonal antibody to V-ATPase F (ATPase, H+ transporting, lysosomal 14 kDa, V1 subunit F)
V-ATPase F antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human V-ATPase F. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product ATP6V1F Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: U87-MG
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
- Alternative Name
- ATPase H+ transporting V1 subunit F (ATP6V1F Products)
- Synonyms
- Atp6s14 antibody, Vma7 antibody, atp6s14 antibody, atp6v1f antibody, vatf antibody, ATP6S14 antibody, VATF antibody, 1110004G16Rik antibody, ATPase H+ transporting V1 subunit F antibody, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog antibody, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog antibody, ATPase, H+ transporting, lysosomal V1 subunit F antibody, Atp6v1f antibody, atp6v1f.S antibody, ATP6V1F antibody, atp6v1f.L antibody
- Background
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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
- Molecular Weight
- 13 kDa
- Gene ID
- 9296
- UniProt
- Q16864
- Pathways
- Transition Metal Ion Homeostasis, Proton Transport
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