ILK antibody

Catalog No. ABIN2854345

Product Details anti-ILK Antibody

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to CKR5 (integrin-linked kinase)
ILK antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , Neuro2A , C8D30 , NIH-3T3 , C2Cl2 , PC-12 , Neuro 2A , C8D30 , Raw264.7 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ILK antibody (ABIN2854345)

Yen, Hsiao, Jiang, Chang, Wang, Shen, Chen, Chang, Chang, Chen et al.: "Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin ..." in: Oncotarget, Vol. 6, Issue 39, pp. 41837-55, (2016) (PubMed).

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: integrin linked kinase (ILK Products)

Synonyms: CG10504 antibody, CT29478 antibody, DmILK antibody, Dmel\\CG10504 antibody, ILK antibody, ilk antibody, l(3)78Ca antibody, p59 antibody, AA511515 antibody, ESTM24 antibody, zgc:65842 antibody, ILK-1 antibody, ILK-2 antibody, P59 antibody, p59ILK antibody, Integrin linked kinase antibody, integrin linked kinase antibody, integrin linked kinase S homeolog antibody, integrin-linked protein kinase antibody, integrin-linked kinase antibody, Ilk antibody, ILK antibody, ilk.S antibody, CpipJ_CPIJ016644 antibody, Bm1_20815 antibody, Tsp_07213 antibody, Tsp_07224 antibody, Tsp_15354 antibody, ilk antibody

Background: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Cell junction , focal adhesion , Cell membrane, Peripheral membrane protein, Cytoplasmic side

Molecular Weight: 51 kDa

Gene ID: 3611

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration