NEFL antibody
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- Target See all NEFL Antibodies
- NEFL (Neurofilament, Light Polypeptide (NEFL))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NEFL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to 68 kDa Neurofilament Light (neurofilament, light polypeptide)
68 kDa Neurofilament Light antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human NF-L. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product NEFL Primary Antibody
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- Application Notes
- WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: PC-3 , U87-MG , SK-N-SH , mouse cerebellum , human brain
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- NEFL (Neurofilament, Light Polypeptide (NEFL))
- Alternative Name
- neurofilament light (NEFL Products)
- Background
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Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
- Molecular Weight
- 62 kDa
- Gene ID
- 4747
- UniProt
- P07196
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