TWIST1 antibody (Center)

Catalog No. ABIN2854290

Product Details anti-TWIST1 Antibody

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Chicken, Cow (Bovine)

Cross-Reactivity (Details): Chicken (82 %), Bovine (97 %)

Characteristics: Rabbit Polyclonal antibody to TWIST1 (twist homolog 1 (Drosophila))
Twist1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Twist1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:5000-1:20000*

Comment:

Positive Control: Flag-human TWIST1-transfected HeLa cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TWIST1 antibody (ABIN2854290)

Lee, Chen, Chen, Yang, Huang, Liu: "Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180383, (2017) (PubMed).

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: Twist1 (TWIST1 Products)

Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Cellular Localization: Nucleus

Molecular Weight: 21 kDa

Gene ID: 7291

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity