POLE3 antibody

Catalog No. ABIN363202

Product Details anti-POLE3 Antibody

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Reacts with DNA polymerase epsilon from human mouse, monkey, mink and hamster

Cross-Reactivity (Details): Does not recognize DNA pol alpha, beta or delta. Does not reacts with Drosophilia or baculovirus host cell pol epsilon, and weakly, if at all, on S. cerivisae pol epsilon.

Purification: Purified (protein G)

Immunogen: Human DNA Polymerase epsilon purified from HeLa cells

Clone: 3C5-1

Isotype: IgG2a

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF1:100, IP1:10-1:500, WB5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 16 mg/mL

Buffer: PBS

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: DNA Polymerase epsilon Subunit 3 (POLE3 Products)

Synonyms: NCU04548.1 antibody, GB14966 antibody, zgc:73351 antibody, 1810034K18Rik antibody, YBL1 antibody, CHARAC17 antibody, CHRAC17 antibody, p17 antibody, DNA polymerase epsilon 3, accessory subunit antibody, polymerase (DNA directed), epsilon 3, accessory subunit antibody, DNA polymerase epsilon antibody, DNA polymerase epsilon p17 subunit antibody, DNA polymerase epsilon subunit 3 antibody, polymerase (DNA directed), epsilon 3 (p17 subunit) antibody, POLE3 antibody, pole3 antibody, NCU04548 antibody, CpipJ_CPIJ008666 antibody, Bm1_04865 antibody, Bm1_07285 antibody, CTRG_00708 antibody, LOAG_09571 antibody, LOC726923 antibody, Pole3 antibody

Background: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

Gene ID: 54107

UniProt: Q9NRF9

Pathways: DNA Damage Repair