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GFP antibody

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Mouse Monoclonal 215 unconjugated

Catalog No. ABIN284433

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
176

15

11

9
Aequorea victoria
Host
64

62

35

15

9

5

1

1
Mouse
Compatible Secondaries
Clonality
115

72
Monoclonal
Conjugate
82

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
150

76

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

The monoclonal detects wild type and recombinant GFP. This antibody does not detect enhanced GFP.

Immunogen

GFP antibody was raised in mouse using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

Clone

215

Isotype

IgG2a

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Application Notes

ELISA: 1:200,000, IHC: 1:1,000-1:5,000, WB: 1:3,000-1:30,000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Concentration

Lot specific

Buffer

0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody