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beta Actin antibody

Name: beta Actin antibody
SKU: ABIN654127
Price: 451 USD
Availability: InStock

ACTB Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB23152 unconjugated

Western blot analysis of lysates from A431, HepG2 cell line (from left to right), using ACTB Antibody . (beta Actin antibody)

ACTB Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). (beta Actin antibody)

FACS

ACTB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (beta Actin antibody)

IHC (p)

3 images

(2 references)

Catalog No. ABIN654127

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Reactivity
177

131

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Human
Host
112

81

5

1

1
Rabbit
Compatible Secondaries
Clonality
111

87
Polyclonal
Conjugate
126

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
167

74

52

46

32

31

25

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Specificity

This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.

Predicted Reactivity

Cow (Bovine),Chicken,Mouse (Murine),Pig (Porcine),Monkey,Rat (Rattus),Xenopus laevis

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.

Clone

RB23152

Isotype

Ig Fraction

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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey WB, IF Host: Goat Polyclonal unconjugated

Application Notes

WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

PBS with 0.09 % (W/V) sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Liu, Peng, Zhong, Liu, Song, Wang: "Effect of 5-caffeoylquinic acid on the NF-?B signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague-Dawley rat adipose tissue." in: Food & function, Vol. 6, Issue 8, pp. 2779-86, (2015) (PubMed).

Shrestha, Mousa, Heintz: "Layer 2/3 pyramidal cells in the medial prefrontal cortex moderate stress induced depressive behaviors." in: eLife, Vol. 4, (2015) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

ACTB (ACTB Products)

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Synonyms: Actin, cytoplasmic 1,ACTB,

Molecular Weight

41737 DA

Gene ID

60

NCBI Accession

NP_001092

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction