FGFR1/FGFR2 antibody (pTyr463, pTyr466) (AbBy Fluor® 594)

Catalog No. ABIN2813231

Product Details anti-FGFR1/FGFR2 Antibody (AbBy Fluor® 594)

Target: FGFR1/FGFR2 (FGFR1, FGFR2)

Binding Specificity: pTyr463, pTyr466

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGFR1/FGFR2 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: This phosphorylation site is found at Tyr463 in Human and Mouse FGFR1, and Tyr486 in Rat. It is highly conserved and is homologous to the Tyr466 phosphorylation site in Human and Mouse FGFR2, and the Tyr370 site in Rat FGFR2.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FGFR1/FGFR2

Target: FGFR1/FGFR2 (FGFR1, FGFR2)

Alternative Name: FGFR1/FGFR2 (FGFR1/FGFR2 Products)

Background:

Synonyms: bFGF R, BFGFR, C FGR, CD 331, CD331, CD331 antigen, CEK, FGFBR, FGFR 1, Fibroblast growth factor receptor 1, FLG, FLG protein, FLJ14326, FLT 2, FLT2, Fms like tyrosine kinase 2, Fms related tyrosine kinase 2, Fms related tyrosine kinase 2 Pfefer syndrome, H2, H3, H4, H5, HBGFR, Heparin binding growth factor receptor, Hydroxyaryl protein kinase, KAL 2, KAL2, MFR, N SAM, N sam tyrosine kinase, Protein tyrosine kinase, Tyrosylprotein kinase, Basic fibroblast growth factor receptor 1.

Background: Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.

Gene ID: 2260, 2263