ARFGEF1 antibody (AA 1-200) (AbBy Fluor® 594)

Catalog No. ABIN2810327

Product Details anti-ARFGEF1 Antibody (AbBy Fluor® 594)

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Binding Specificity: AA 1-200

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGEF1 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARFGEF1

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Alternative Name: BIG1/ARFGEF1 (ARFGEF1 Products)

Synonyms: ARFGEF1 antibody, ARFGEP1 antibody, BIG1 antibody, P200 antibody, D130059B05Rik antibody, D730028O18Rik antibody, RGD1560793 antibody, ADP ribosylation factor guanine nucleotide exchange factor 1 antibody, brefeldin A-inhibited guanine nucleotide-exchange protein 1 antibody, ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) antibody, ARFGEF1 antibody, CpipJ_CPIJ004831 antibody, Arfgef1 antibody

Background:

Synonyms: ADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited, ARFGEF1, ARFGEP1, Brefeldin A inhibited GEP 1, Brefeldin A inhibited guanine nucleotide exchange protein 1, p200 ARF GEP1, p200 ARF guanine nucleotide exchange factor, BIG1_HUMAN.

Background: Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.

Gene ID: 10565

Pathways: Regulation of Actin Filament Polymerization, Regulation of Carbohydrate Metabolic Process