CDK7 antibody (Middle Region)

Catalog No. ABIN504682

Product Details anti-CDK7 Antibody

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Pig, Horse, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK7 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TQALKMKYFS NRPGPTPGCQ LPRPNCPVET LKEQSNPALA IKRKRTEALE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Cow: 80%, Dog: 86%, Guinea Pig: 78%, Horse: 86%, Human: 100%, Pig: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against CDK7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CDK7

Application Details

Application Notes: WB Suggested Anti-CDK7 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:12500
Positive Control: THP-1 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 346 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDK7

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Alternative Name: CDK7 (CDK7 Products)

Background: CDK7 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAK1, CDKN7, MO15, STK1, p39MO15, HCAK
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, GTF2H4, GTF2H3, GTF2H2, GTF2H1, ERCC5, ERCC3, ERCC2, CDK7, CCNH, ATP2B4, A2M, GTF2H5, SRPK2, SRPK1, MNAT1, TP53, CDK2, CTD, MBP, CDK1, CCNB1, CCNA2, Dlg4, UVSSA, HSP90AA1, HSD17B4, HLA-DQA1, SUPT5H, POLR2A, GTF2E2, GTF2E1, CDK9, APP
Protein Size: 346

Molecular Weight: 39 kDa

Gene ID: 1022

NCBI Accession: NM_001799, NP_001790

UniProt: P50613

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, M Phase