GTF2B antibody (N-Term)

Catalog No. ABIN2792692

Product Details anti-GTF2B Antibody

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA IASACLYIAC RQEGVPRTFK

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against GTF2B. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2B

Application Details

Application Notes: WB Suggested Anti-GTF2B Antibody Titration: 4.0 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 316 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GTF2B antibody (ABIN2792692)

Tubon, Tansey, Herr: "A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2863-74, (2004) (PubMed).

Target Details for GTF2B

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: GTF2B (GTF2B Products)

Synonyms: CG5193 antibody, Dmel\\CG5193 antibody, TFIIB antibody, Tfiib antibody, TfllB antibody, dTFIIB antibody, dTfIIB antibody, dmTFIIB antibody, gtf2b antibody, tf2b antibody, tfiib antibody, XLTFIIB antibody, TF2B antibody, wu:fc44c11 antibody, zgc:66258 antibody, Transcription factor IIB antibody, transcription initiation factor iib antibody, transcription initiation factor IIB antibody, transcription initiation factor IIB 2 antibody, Transcription initiation factor IIB antibody, general transcription factor 2B S homeolog antibody, general transcription factor 2B L homeolog antibody, general transcription factor IIB antibody, TfIIB antibody, CNF01950 antibody, tfb antibody, CC1G_14689 antibody, HacjB3_18813 antibody, HALXA_RS07110 antibody, HALXA_RS16310 antibody, tf2b antibody, LOC100283348 antibody, gtf2b.S antibody, gtf2b.L antibody, GTF2B antibody, gtf2b antibody, Gtf2b antibody

Background: GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
Alias Symbols: TF2B, TFIIB
Protein Interaction Partner: DRAP1, TNIP1, UBC, FBXO25, HNF4A, TCEA1, GTF2F2, GTF2F1, DEPDC7, SLC22A2, RELA, POLR2C, JAK3, HMGCL, SMURF1, THRA, TBP, POLR2E, SRA1, LSM4, LSM5, CIITA, NXF1, POU3F2, EP300, PSMA3, POLR2J, ESR1, UL48, NR1D2, NCOR1, TAF9, REST, tat, SAP25, MKX, CTDP1, POLR
Protein Size: 316

Molecular Weight: 35 kDa

Gene ID: 2959

NCBI Accession: NM_001514, NP_001505

UniProt: Q00403