SUB1 antibody (Middle Region)

Catalog No. ABIN2792675

Product Details anti-SUB1 Antibody

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NMFQIGKMRY VSVRDFKGKV LIDIREYWMD PEGEMKPGRK GISLNPEQWS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PC4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PC4

Application Details

Application Notes: WB Suggested Anti-PC4 Antibody Titration: 0.5-1.0 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 127 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: SUB1 / PC4 (SUB1 Products)

Background: PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.
Alias Symbols: P15, PC4, p14
Protein Interaction Partner: HUWE1, SUB1, UBC, rev, CLK4, CDK2, HNF4A, FN1, CSNK2A1, SMURF1, RPL18A, LAMP2, CDC5L, APP, YWHAZ, GTF3C1, ELAVL1, SUMO2, HDGF, Shoc2, Kif1c, POLR2B, POLR2A, CBX5, RCOR1, REST, BANF1, ADAP1, GTF2A1L, EP300, POU2AF1, CSTF2, TBP, TAF1, PCNA, HSF1, PSIP1, SP1
Protein Size: 127

Molecular Weight: 14 kDa

Gene ID: 10923

NCBI Accession: NM_006713, NP_006704

UniProt: P53999