LEF1 antibody (N-Term)
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- Target See all LEF1 Antibodies
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LEF1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN
- Cross-Reactivity
- Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
- Predicted Reactivity
- Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 85%, Rat: 92%
- Characteristics
- This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1
- Top Product
- Discover our top product LEF1 Primary Antibody
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- Application Notes
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WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Comment
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Antigen size: 399 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
- Alternative Name
- LEF1 (LEF1 Products)
- Synonyms
- LEF1 antibody, 3000002B05 antibody, AI451430 antibody, Lef-1 antibody, LEF-1 antibody, TCF10 antibody, TCF1ALPHA antibody, TCF7L3 antibody, lymphoid enhancer binding factor 1 antibody, lymphoid enhancer-binding factor 1 antibody, lymphoid enhancer factor XLEF-1B antibody, LEF1 antibody, lef1 antibody, Lef1 antibody
- Background
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Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399 - Molecular Weight
- 44 kDa
- Gene ID
- 51176
- NCBI Accession
- NM_016269, NP_057353
- UniProt
- Q9UJU2
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
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