NFIC antibody (C-Term)

Catalog No. ABIN2792601

Product Details anti-NFIC Antibody

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIC antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VRERDAEQSG SPRTGMGSDQ EDSKPITLDT TDFQESFVTS GVFSVTELIQ

Cross-Reactivity: Human

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against NFIon Channel. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NFIC

Application Details

Application Notes: WB Suggested Anti-NFIC Antibody Titration: 2.5 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 428 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFIC antibody (ABIN2792601)

Liang, Tikhanovich, Nasheuer, Folk: "Stimulation of BK virus DNA replication by NFI family transcription factors." in: Journal of virology, Vol. 86, Issue 6, pp. 3264-75, (2012) (PubMed).

Target Details for NFIC

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Alternative Name: NFIC (NFIC Products)

Synonyms: LOC100005568 antibody, 1110019L22Rik antibody, 1500041O16Rik antibody, AA589446 antibody, AI746521 antibody, NF1-C antibody, CTF antibody, CTF5 antibody, NF-I antibody, NFI antibody, CNFI-C antibody, nf1-c1 antibody, nuclear factor I/C antibody, nuclear factor I C antibody, nuclear factor I C L homeolog antibody, nfic antibody, NFIC antibody, Nfic antibody, nfic.L antibody

Background: Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.
Alias Symbols: CTF, NFI, CTF5, NF-I
Protein Interaction Partner: HSP90AA1, TFAP4, Cebpb, ELAVL1, SUMO2, UBC, SMURF1, SMAD3, ZCCHC14, ZKSCAN7, TPD52L3, TKTL2, LLPH, NFIB, GLRX, CREBBP, RFX1, TLX1,
Protein Size: 428

Molecular Weight: 48 kDa

Gene ID: 4782

NCBI Accession: NM_005597, NP_005588

UniProt: P08651