NFX1 antibody (Middle Region)

Catalog No. ABIN2792551

Product Details anti-NFX1 Antibody

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 85%, Dog: 85%, Human: 100%, Mouse: 76%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFX1

Application Details

Application Notes: WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1120 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Alternative Name: NFX1 (NFX1 Products)

Synonyms: NFX1 antibody, NFX2 antibody, 1300017N15Rik antibody, 3000003M19Rik antibody, NFX.1 antibody, TEG-42 antibody, Tex42 antibody, nuclear transcription factor, X-box binding 1 antibody, nuclear transcription factor, x-box binding 1 antibody, NFX1 antibody, CpipJ_CPIJ011986 antibody, nfx1 antibody, Nfx1 antibody

Background: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Alias Symbols: DKFZp779G2416, MGC20369, NFX2
Protein Interaction Partner: UBC, BMI1, HECW2, SRSF4, SRSF3, RPS19, RPS3A, RPL22, RPL17, RPL13, RPL7A, RPL6, RPL3, PABPC3, YBX1, NCL, HNRNPM, HSPA1A, HNRNPU, HNRNPH1, HNRNPA2B1, HIST1H2AE, EEF1A2, DMD, DDX5, FAM208B, PABPC1, RPL13A, LARP1, APC2, MATR3, HIST1H4E, USP9X, SIN3A, MAML3,
Protein Size: 1120

Molecular Weight: 124 kDa

Gene ID: 4799

NCBI Accession: NM_002504, NP_002495

UniProt: Q12986