DEK antibody (N-Term)

Catalog No. ABIN2792543

Product Details anti-DEK Antibody

Target: DEK (DEK Oncogene (DEK))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEK antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AAEGEGTPTQ PASEKEPEMP GPREESEEEE DEDDEEEEEE EKEKSLIVEG

Cross-Reactivity: Cow (Bovine), Human, Rat (Rattus)

Predicted Reactivity: Cow: 78%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against DEK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DEK

Application Details

Application Notes: WB Suggested Anti-DEK Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 375 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DEK antibody (ABIN2792543)

Wang, Eckert, Zomorrodi, Xin, Pan, Shearer, Weisz, Maranus, Clawson: "Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways." in: PLoS ONE, Vol. 7, Issue 6, pp. e39446, (2012) (PubMed).

Target Details for DEK

Target: DEK (DEK Oncogene (DEK))

Alternative Name: DEK (DEK Products)

Synonyms: D6S231E antibody, DEK antibody, 1810019E15Rik antibody, D13H6S231E antibody, DEK proto-oncogene antibody, DEK proto-oncogene L homeolog antibody, DEK oncogene (DNA binding) antibody, DEK oncogene antibody, DEK antibody, Dek antibody, dek.L antibody, LOC398543 antibody

Background: DEK was first identified in a fusion with the CAN nucleoporin protein in a specific subtype of acute myelogenous leukemia. DEK has also been shown to be an autoantigen in patients with pauciarticular onset juvenile rheumatoid arthritis. Further, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia
Alias Symbols: D6S231E
Protein Interaction Partner: CUL3, SPOP, UBC, EED, IRF2BP2, GANAB, PTPN12, CSNK2A2, CSNK2A1, TP53BP1, ESR1, VTN, S100A9, RPL37A, DHX15, FTSJ3, WIBG, TMED9, EXOSC2, UTP14A, THRAP3, CAND1, COPS5, CDK2, YWHAZ, Cebpb, ELAVL1, SUMO1, SUMO2, RAD21, HDGF, KAT2B, EP300, CREBBP, HIST1H4A, HDA
Protein Size: 375

Molecular Weight: 43 kDa

Gene ID: 7913

NCBI Accession: NM_003472, NP_003463

UniProt: P35659