FFAR1 antibody (N-Term)

Catalog No. ABIN2792205

Product Details anti-FFAR1 Antibody

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KVSEAALSLL VLILIITSAS RSQPKVPEWV NTPSTCCLKY YEKVLPRRLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 85%, Pig: 92%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against FFAR1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FFAR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 300 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FFAR1 antibody (ABIN2792205)

Sum, Tikhonova, Neumann, Engel, Raaka, Costanzi, Gershengorn: "Identification of residues important for agonist recognition and activation in GPR40." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29248-55, (2007) (PubMed).

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: FFAR1 (FFAR1 Products)

Background: FFAR1 is receptor for medium and long chain saturated and unsaturated fatty acids. It binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C.
Alias Symbols: FFA1R, GPCR40, GPR40
Protein Interaction Partner: GNAQ, GNAI1,
Protein Size: 300

Molecular Weight: 31 kDa

Gene ID: 2864

NCBI Accession: NM_005303, NP_005294

UniProt: O14842

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis