PCNA antibody (C-Term)

Catalog No. ABIN2792157

Product Details anti-PCNA Antibody

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LNFFTKATPL SSTVTLSMSA DVPLVVEYKI ADMGHLKYYL APKIEDEEGS

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PCNA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PCNA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 261 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PCNA antibody (ABIN2792157)

Liu, Meng, Li, Zhou, Zeng, He, Mrsny, Liu, Hu, Hu, Li: "Human umbilical cord mesenchymal stromal cells rescue mice from acetaminophen-induced acute liver failure." in: Cytotherapy, Vol. 16, Issue 9, pp. 1207-19, (2014) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Background: PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Alias Symbols: MGC8367
Protein Interaction Partner: GADD45A, CDKN1A, HUWE1, POLH, PCNA, MSH3, FUS, UBC, TMEM218, CHFR, DTL, POLE, POLD1, MCM7, MCM4, LIG1, MSH6, FEN1, FBXO18, ZRANB3, CHTF18, DNMT1, RAD18, SIVA1, KIAA0101, TDG, SUMO2, SUMO3, RPA1, RFC5, RFC4, RFC3, RFC2, RFC1, MDM2, TRIM25, USP18, ISG15, MA
Protein Size: 261

Molecular Weight: 29 kDa

Gene ID: 5111

NCBI Accession: NM_002592, NP_002583

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy