YWHAE antibody (Middle Region)

Catalog No. ABIN2792142

Product Details anti-YWHAE Antibody

Target: YWHAE (14-3-3 epsilon (YWHAE))

Binding Specificity: Middle Region

Reactivity: Mouse, Zebrafish (Danio rerio), Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ELDTLSEESY KDSTLIMQLL RDNLTLWTSD MQGDGEEQNK EALQDVEDEN

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Mouse: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against YWHAE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human YWHAE

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 255 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-YWHAE antibody (ABIN2792142)

Tak, Jang, Kim, Park, Suk, Yoon, Ahn, Lee, Joe: "14-3-3epsilon inhibits MK5-mediated cell migration by disrupting F-actin polymerization." in: Cellular signalling, Vol. 19, Issue 11, pp. 2379-87, (2007) (PubMed).

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE (YWHAE Products)

Background: YWHAE is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. YWHAE binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. The binding generally results in the modulation of the activity of the binding partner.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: 14-3-3E, FLJ45465, KCIP-1, MDCR, MDS
Protein Interaction Partner: DTL, HUWE1, FAM53C, FBXW11, SORBS2, UBC, ABL1, GRB10, SIK2, CEP57, SIK3, TP53, IRS4, CBL, TUBB2A, TWF2, PROSC, FERMT2, PDIA4, RAP1GDS1, PTMA, PPP1R2, PDHB, PAK2, PAFAH1B2, YWHAH, XPO1, VCL, GSS, G6PD, EIF5, TBCB, CAPNS1, CAPN2, ALDH7A1, ATIC, ASNS, SCPEP1
Protein Size: 255

Molecular Weight: 29 kDa

Gene ID: 7531

NCBI Accession: NM_006761, NP_006752

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase