FLIP antibody (Middle Region)

Catalog No. ABIN504572

Product Details anti-FLIP Antibody

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LNPEGGKSGK APRRRAASMD SSSKLLRGRS KAPKKKPSVL PAPPEGATPT

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against CFLAR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CFLAR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 480 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR (CFLAR Products)

Background: CFLAR belongs to the peptidase C14A family. It is a apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. The protein acts as an inhibitor of TNFRSF6 mediated apoptosis. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.
Alias Symbols: CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLIP, I-FLICE, MRIT, USURPIN, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, FLAME1
Protein Interaction Partner: FADD, TRAF1, GSN, SPATS1, TNFSF10, RIPK1, RAF1, SMAD3, CASP8, HSP90AA1, STUB1, TNFRSF10A, TNFRSF10B, UBC, FASLG, ITCH, DAXX, CBLB, ATG3, XRCC6, Mib1, TP53, DEDD2, DEDD, RIPK2, IKBKB, MAP2K7, TRAF2, CASP10, CASP7, CASP6, MAP2K4, PSEN2, CASP2, CASP3, BCL2L1
Protein Size: 480

Molecular Weight: 55 kDa

Gene ID: 8837

NCBI Accession: NM_003879, NP_003870

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development