RRP8 antibody (N-Term)

Catalog No. ABIN2791071

Product Details anti-RRP8 Antibody

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRP8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GKKGKKKCQK QGPPCSDSEE EVERKKKCHK QALVGSDSAE DEKRKRKCQK

Predicted Reactivity: Cow: 83%, Horse: 85%, Human: 100%, Rabbit: 85%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RRP8. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human RRP8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 456 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RRP8

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Alternative Name: RRP8 (RRP8 Products)

Synonyms: KIAA0409 antibody, 1500003O22Rik antibody, 2900001K19Rik antibody, AW538116 antibody, RGD1308302 antibody, ribosomal RNA processing 8 antibody, ribosomal RNA processing 8, methyltransferase, homolog (yeast) antibody, RRP8 antibody, Rrp8 antibody

Background: RRP8 is an essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Alias Symbols: KIAA0409
Protein Interaction Partner: RNF2, SIRT7, SIRT1, SUV39H1, USP39,
Protein Size: 456

Molecular Weight: 50 kDa

Gene ID: 23378

NCBI Accession: NM_015324, NP_056139

UniProt: O43159