PYM antibody (C-Term)

Catalog No. ABIN2788485

Product Details anti-PYM Antibody

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow, Dog, Horse, Guinea Pig, Rabbit, Rat, Saccharomyces cerevisiae, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYM antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KKIKNLKKKL RQVEELQQRI QAGEVSQPSK EQLEKLARRR ALEEELEDLE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against WIBG. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human WIBG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 204 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYM

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Alternative Name: WIBG (WIBG Products)

Synonyms: BEST:HL05757 antibody, CG10330 antibody, CG30176 antibody, Dmel\\CG30176 antibody, PYM antibody, Pym antibody, UD3 antibody, A030010B05Rik antibody, zgc:65891 antibody, zgc:77281 antibody, Partner of Y14 and Mago antibody, Wibg protein antibody, PYM homolog 1, exon junction complex associated factor L homeolog antibody, PYM homolog 1, exon junction complex associated factor antibody, Pym antibody, wibg antibody, pym1.L antibody, PYM1 antibody, Pym1 antibody, pym1 antibody

Background: WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. It acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. It interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions.
Alias Symbols: MGC13064, PYM
Protein Interaction Partner: RTN4IP1, UBC, RPS19, RPS14, RPS13, RPS12, RPS10, RPS9, RPS8, RPS7, RPS6, RPS5, RPS4X, RPS3A, RPS3, RPS2, FAU, BYSL, ZNF503, PNO1, KCMF1, TSR1, RPS27L, UBQLN1, RPS29, RPS28, RPS27, RPS26, RPS25, RPS24, RPS23, RPS20, RPS18, RPS16, RPS15A, TARDBP, MAGOH, TOE
Protein Size: 204

Molecular Weight: 23 kDa

Gene ID: 84305

NCBI Accession: NM_032345, NP_115721

UniProt: Q9BRP8