RPS27A antibody (Middle Region)

Catalog No. ABIN2788060

Product Details anti-RPS27A Antibody

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Sheep, Guinea Pig, Dog, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS27A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LRGGAKKRKK KSYTTPKKNK HKRKKVKLAV LKYYKVDENG KISRLRRECP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RPS27A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS27A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 156 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPS27A

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Alternative Name: RPS27A (RPS27A Products)

Background: Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin, similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
Alias Symbols: CEP80, UBA80, UBCEP1, UBCEP80, UBC, S27A
Protein Interaction Partner: CALCOCO2, DAZAP2, UBC, MDM2, TRIM25, EED, TP53, RNF11, rev, TUBA4A, PARK2, CDK2, PAN2, ZNF512B, SMAD2, ITGA4, FN1, CDK11B, UBL4A, VCAM1, RPL21, RPL19, RPL18A, RPL18, RPL17, RPL15, RPL11, RPL9, RPL7A, RPL6, RPL5, RPL4, RPL37A, RPL31, RPL24, RPL23A, RPL22,
Protein Size: 156

Molecular Weight: 18 kDa

Gene ID: 6233

NCBI Accession: NM_002954, NP_002945

UniProt: P62979

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation