NAE1 antibody (C-Term)
-
- Target See all NAE1 Antibodies
- NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))
-
Binding Specificity
- C-Term
- Reactivity
- Human, Mouse, Rat, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This NAE1 antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
- Characteristics
- This is a rabbit polyclonal antibody against Nae1. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1
- Top Product
- Discover our top product NAE1 Primary Antibody
-
-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Antigen size: 534 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))
- Alternative Name
- Nae1 (NAE1 Products)
- Synonyms
- APPBP1 antibody, CG7828 antibody, Dmel\\CG7828 antibody, anon-WO0140519.225 antibody, dAPP-BP1 antibody, dAPPBP1 antibody, A-116A10.1 antibody, ula-1 antibody, Appbp1 antibody, 59kDa antibody, appbp1 antibody, zgc:66148 antibody, beta-Amyloid precursor protein binding protein 1 antibody, NEDD8 activating enzyme E1 subunit 1 antibody, NEDD8 activating enzyme E1 subunit 1 S homeolog antibody, nedd8 activating enzyme E1 subunit 1 antibody, NEDD8-activating enzyme E1 regulatory subunit antibody, APP-BP1 antibody, NAE1 antibody, Nae1 antibody, nae1.S antibody, nae1 antibody, ula-1 antibody
- Background
-
Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Alias Symbols: 59 kDa, Appbp1
Protein Size: 534 - Molecular Weight
- 60 kDa
- Gene ID
- 234664
- NCBI Accession
- NM_144931, NP_659180
- UniProt
- Q8VBW6
-