USP8 antibody (C-Term)

Catalog No. ABIN2788008

Product Details anti-USP8 Antibody

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: FAGYSQQDSQ ELLLFLMDGL HEDLNKADNR KRYKEENNDH LDDFKAAEHA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against USP8. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human USP8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1118 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for USP8

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Alternative Name: USP8 (USP8 Products)

Synonyms: HumORF8 antibody, UBPY antibody, AI574262 antibody, AW557536 antibody, Ubpy antibody, mKIAA0055 antibody, ubiquitin carboxyl-terminal hydrolase 8 antibody, ubiquitin specific peptidase 8 antibody, LOC9310109 antibody, MGYG_00369 antibody, USP8 antibody, Usp8 antibody

Background: USP8 is a hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. USP8 catalytic activity is enhanced in the M phase. USP8 is involved in cell proliferation. USP8 is required to enter into S phase in response to serum stimulation. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. USP8 probably regulates the stability of STAM2 and RASGRF1. USP8 regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle.USP8 deubiquitinates EPS15 and controles tyrosine kinase stability.USP8 removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. USP8 is involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.
Alias Symbols: FLJ34456, HumORF8, KIAA0055, MGC129718, UBPY
Protein Interaction Partner: VAMP8, LRIG1, TARDBP, UBC, PTEN, STAT6, TSEN2, POLD3, HTT, YWHAB, YWHAQ, YWHAE, EGFR, PTPN23, SRC, ERBB4, UBE2I, UBE2G1, FARSB, VPS4B, TRIM63, TRIM55, PJA1, USP28, TRIM54, USP21, USP15, GRAP2, CHMP4C, CHMP1B, CHMP2A, CHMP2B, RNF41, USP8, SPP1, CHMP1A, NBR
Protein Size: 1118

Molecular Weight: 127 kDa

Gene ID: 9101

NCBI Accession: NM_005154, NP_005145

UniProt: P40818