USP7 antibody (Middle Region)

Catalog No. ABIN2787924

Product Details anti-USP7 Antibody

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP7 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RVLLDNVENK MKGTCVEGTI PKLFRGKMVS YIQCKEVDYR SDRREDYYDI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against USP7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human USP7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1102 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-USP7 antibody (ABIN2787924)

Nie, Zhang, Dai, Zhang, Zhao, Xu, Chen, Wang, Wang, Qiao: "Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation." in: Biology of reproduction, Vol. 94, Issue 2, pp. 31, (2016) (PubMed).

Target Details for USP7

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Alternative Name: USP7 (USP7 Products)

Synonyms: CG1490 antibody, Dmel\\CG1490 antibody, USP7 antibody, 2210010O09Rik antibody, AA409944 antibody, AA617399 antibody, AU019296 antibody, AW548146 antibody, C80752 antibody, Hausp antibody, HAUSP antibody, TEF1 antibody, UBP antibody, Ubiquitin-specific protease 7 antibody, ubiquitin carboxyl-terminal hydrolase 7 antibody, ubiquitin specific peptidase 7 antibody, Usp7 antibody, LOC100201254 antibody, USP7 antibody

Background: USP7 is a hydrolase that deubiquitinates target proteins such as FOXO4, TP53, MDM2, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and proteasomal degradation. USP7 deubiquitinates TP53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, USP7 is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 is involved in cell proliferation during early embryonic development. USP7 contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.
Alias Symbols: HAUSP, TEF1
Protein Interaction Partner: UBC, TP53, CHFR, RNF220, TPP1, FAM175B, HUWE1, PEPD, XPC, SUMO2, IKBKG, SHFM1, RB1, SUMO1, RPA3, RPA2, RPA1, PTEN, EED, ABL1, RNF2, MMS19, PPP6C, FKBP5, PPP6R3, CDC37, OGFR, COPS6, MAGED2, TRAP1, MCMBP, CLK3, UHRF1, PPARG, RNF11, KAT5, SOX2, LOC576779, HD
Protein Size: 1102

Molecular Weight: 128 kDa

Gene ID: 7874

NCBI Accession: NM_003470, NP_003461

UniProt: Q93009