Nestin antibody (Middle Region)

Catalog No. ABIN2787826

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: Middle Region

Reactivity: Human, Dog, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LPDSTPLGFY LRSPTSPRWD PTGEQRPPPQ GETGKEGWDP AVLASEGLEA

Predicted Reactivity: Cow: 79%, Dog: 79%, Horse: 86%, Human: 100%, Pig: 86%

Characteristics: This is a rabbit polyclonal antibody against NES. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NES

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1621 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES (NES Products)

Background: Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neu
Alias Symbols: FLJ21841, Nbla00170
Protein Interaction Partner: SUMO3, UBC, SIRT7, CDK5R1, CDK5, SRRM1, CDK1,
Protein Size: 1621

Molecular Weight: 177 kDa

Gene ID: 10763

NCBI Accession: NM_006617, NP_006608

UniProt: P48681