TUFM antibody (Middle Region)

Catalog No. ABIN2787734

Product Details anti-TUFM Antibody

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Rabbit, Saccharomyces cerevisiae, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUFM antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PEKELAMPGE DLKFNLILRQ PMILEKGQRF TLRDGNRTIG TGLVTNTLAM

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 93%, Yeast: 77%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against TUFM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TUFM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 455 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TUFM antibody (ABIN2787734)

Clemente, Peralta, Cruz-Bermudez, Echevarría, Fontanesi, Barrientos, Fernandez-Moreno, Garesse: "hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8321-31, (2013) (PubMed).

Target Details for TUFM

Target: TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))

Alternative Name: TUFM (Tufm Products)

Synonyms: TUFM antibody, D250 antibody, fi06f04 antibody, wu:fi06f04 antibody, zgc:110766 antibody, COXPD4 antibody, EF-TuMT antibody, EFTU antibody, P43 antibody, 2300002G02Rik antibody, C76308 antibody, C76389 antibody, Tu translation elongation factor, mitochondrial antibody, TUFM antibody, tufm antibody, Tufm antibody

Background: TUFM is a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: COXPD4, EF-TuMT, EFTU, P43
Protein Interaction Partner: FAM9B, FUNDC1, CMTM5, ARL6IP1, HUWE1, FUS, SUMO2, SUMO3, SPRTN, UBC, MDM2, ASB12, ASB14, ASB10, ASB17, SUZ12, BMI1, RNF2, TUBB2A, XPO1, TUBB6, CAMK1D, NSFL1C, IPO9, UBXN1, DCPS, PROSC, PDIA4, PLAA, GTF3C4, NAE1, PPP5C, PPP1R2, PAFAH1B2, HK1, HEXB, GAPDH,
Protein Size: 455

Molecular Weight: 50 kDa

Gene ID: 7284

NCBI Accession: NM_003321, NP_003312

UniProt: P49411