MED14 antibody (Middle Region)

Catalog No. ABIN2787568

Product Details anti-MED14 Antibody

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED14 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Sequence: AADREDSPAM ALLLQQFKEN IQDLVFRTKT GKQTRTNAKR KLSDDPCPVE

Predicted Reactivity: Human: 100%, Mouse: 79%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against MED14. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED14

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1454 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED14

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Alternative Name: MED14 (MED14 Products)

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
Alias Symbols: CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, MGC104513, RGR1, TRAP170
Protein Interaction Partner: UBC, HECW2, CDK8, CDK19, MED12, ESR1, MED19, MED26, PPARG, FBXW7, EPAS1, MED11, MED8, MED29, MED18, MED4, PPP6R1, MED16, MED13, MED24, MED27, MED17, MED21, MED1, CTDP1, SREBF1, DCTN1, ACTN2, ACTN1, MED10, UACA, NECAB2, MED25, MED15, CRSP5, MED7, MED23, VD
Protein Size: 1454

Molecular Weight: 160 kDa

Gene ID: 9282

NCBI Accession: NM_004229, NP_004220

UniProt: O60244

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha